A practice-changing culture method relying on shaking substantially increases mitochondrial energy metabolism and functionality of human liver cell lines.

PLoS One · 2018 · PMC5908182 · PMID 29672606

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In addition, DMF-culturing increased hepatic differentiation, as indicated by a 1.9-,3.1- and 5.6-fold induction of the transcript levels of HNF4 , CAR and CEBPα , while PXR and SHP exhibited a positive trend ( Fig 6C , S3 Table ).

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