High throughput measurement of metabolism in planarians reveals activation of glycolysis during regeneration.

With time after injury, there was a decreasing trend in the OCR:ECAR values, with a significant difference occurring 96 h after injury; however, no significant differences in OCR:ECAR were found after removal of statistical outliers (Figs 3 D and S3 C). 2.4 Effect of metabolic inhibitors on extracellular flux measurements Metabolic inhibitors are commonly used in XF assays to interrogate metabolism in isolated cells (Dranka et al., 2011 ; Hill, Dranka, Zou, Chatham, & Darley‐Usmar, 2009 ; Hill et al., 2012 ).

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