highly significantP <0.001
For the second truss, bif was less branched when exposed to lower temperatures up to and including the third transfer (means of the first three transfers: cold=2.96 ± 0.22; control=4.07 ± 0.21), and the effect was inverted by the fourth and fifth transfers (cold=4.14 ± 0.55; control=2.01 ± 0.23), resulting in a highly significant treatment×transfer point interaction ( P <0.001; Table 2 ).