Differential expression of virulence genes in Legionella pneumophila growing in Acanthamoeba and human monocytes.

Although vipD exhibited 1.8- and 1.3-fold downregulation at 12 and 24 hours, respectively, the expression levels exhibited an increasing trend in the second infection cycle, with 1.8- and 4.6-fold upregulation at 36 and 48 hours, respectively ( Fig. 3 ).

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