Ratiometric analysis revealed a highly significant 3.6 ± 0.3-fold signal amplification following incubation with 1 mM 8-Br-cGMP, whereas higher or lower concentrations were not as efficient (0.5 mM: 2.2 ± 0.2-fold amplification, 2.5 mM: 2.8 ± 0.1-fold amplification; Figure 5 A).
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Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies.
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