Metformin Induces Apoptosis and Alters Cellular Responses to Oxidative Stress in Ht29 Colon Cancer Cells: Preliminary Findings.

The semi-quantitative evaluation of immunostaining intensity (IFIS) in Table 1 showed that the level of NRF-2 and NF-κB proteins had a decreasing trend in a dose- and time-dependent manner, with statistical significance of the different expressions among the groups of treated cells with respect to the untreated ones.

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The semiquantitative evaluation of immunostaining intensity, reported as the Immunofluorescence Intensity Score (IFIS) in Table 1 , showed that the level of cleaved PARP-1, Caspase-3, APAF-1, and MAPLC3 proteins had an increasing trend in a dose- and time-dependent manner, with statistical significance of the different expression among the groups of treated cells, and with respect to untreated cells.

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