an increasing trendP = 0.483
However, there were no significant differences in Th1 and Th2 cells between PM2.5-induced rats and controls, although there was an increasing trend in the frequency of Th2 cells (4.362 ± 2.392, 5.253 ± 1.873, P = 0.483; 4.816 ± 3.453, 1.582 ± 0.798, P = 0.055) (Fig. 3 ).