We next replicated the RNA-seq data for a subset of genes by performing qPCR on the IP and total RNA and reconfirmed highly significant enrichment of Pmch (102.5 Fold), Tacr3 (18.4 Fold), and Cartp (29.9 Fold) ( Figure 1 D).
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Functional analysis reveals differential effects of glutamate and MCH neuropeptide in MCH neurons.
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