Choice of fixative for IHC: We have observed little (almost insignificant) difference in organoid section quality when organoids were fixed in cold acetone or cold PFA.
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Development and Characterization of Human Cerebral Organoids: An Optimized Protocol.
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For example, certain gene mutations that are responsible for severe phenotypes and devastating neurodevelopmental conditions in humans exhibit either no phenotype or a very subtle (barely significant) phenotype in the mouse brain 7 – 10 .