Next, we plotted these values against published K D values determined with cell‐free biochemical methods (Wang et al , 2004 ; Kastritis et al , 2011 ) and observed a highly significant correlation between BRET 50 and published K D values (Fig 3 E).
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LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
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