Validation by RT-PCR Eight genes that showed highly significant differential expression between ST and SS (the most contrasting muscles in terms of metabolic and histochemical properties according to Ithurralde et al., 2015, 2017 [ 12 , 13 ]) were chosen for validation of the RNA-Seq results: myozenin 2 ( MYOZ2 ), myosin light chain 2 ( MYL2 ), myosin light chain 3 ( MYL3 ), tropomyosin 3 ( TPM3 ), myosin light chain 6 ( MYL6 ), troponin T1 ( TNNT1 ), troponin C1 ( TNNC1 ) and methyl transferase-like 21C ( METTL21C ).
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Comparison of transcriptomic landscapes of different lamb muscles using RNA-Seq.
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