highly significantp = 0.002
Our data clearly showed that by 24 h, the GUS transcript level increased by ~ 3.5 fold from 3 h, which was highly significant when compared to 3 and 9 h ( p = 0.002 and 0.008, respectively, Fig. 3 ), an indicator of the improved efficiency of transient transformation, suggesting that extended AS induction time could be a valuable approach to improve large size T-DNA transfer to rice cells.