Transcriptional programming of lipid and amino acid metabolism by the skeletal muscle circadian clock.

Another biomarker of oxidative stress resulting from peroxidation of PUFAs, 4-hydroxy-2-nonenal, also showed only a slight trend for increased abundance during the fasting+rest phase and otherwise remained within a normal diurnal physiological range ( S6A Fig ).

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However, we detected a highly significant 20% reduction in complex II+III activity in mKO muscles, suggesting a mild coenzyme Q deficiency, consistent with reduced Coq10b ( Fig 2B ), whereas complex IV activity was significantly reduced around 30%.

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