Polysaccharide isolated from seeds of <i>Plantago asiatica</i> L. induces maturation of dendritic cells through MAPK and NF-κB pathway.

p < .05 was considered as statistically significant, p < .01 as highly significant. 3 Results 3.1 PLP-2 activates the phosphorylation of MAPK proteins and nuclear translocation of NF-κB in DCs To determine whether MAPK pathway was activated by PLP-2, immature DCs were treated with PLP-2 (100 μg/mL) and LPS (1 μg/mL) respectively for 24 h, and then phosphorylation of MAPK proteins was determined by western blotting.

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