The BMD of gld mice showed an increasing trend (avg gld = 590.779 mg/cm 3 , sd gld = 85.619) vs. wild type mice (avg wt = 529.221 mg/cm 3 , sd wt = 23.988), but the difference was not statistically significant ( p = 0.1208, data not shown).
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These results were further validated at the protein level by an immunofluorescence analysis also showing a decreasing trend in the mutant vs. the wild type mice for the Mmp2 ( Figures 3B,F ) and Sost proteins ( Figures 3C,G ).