Further supporting CO–RUP interaction in yeast, our in planta FRET-FLIM analysis detected highly significant changes in the lifetime of the donor RUP1-GFP and RUP2-GFP fusions in the nucleus when coexpressed with CO-mCherry ( Fig. 3 C).
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<i>Arabidopsis</i> RUP2 represses UVR8-mediated flowering in noninductive photoperiods.
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