During the last few years, there has been an increasing trend towards the use of transient expression of recombinant proteins in plants, mostly because this approach is fast and flexible, allowing the rapid expression of high concentrations of heterologous proteins in a matter of days [ 13 ].
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Minimally processed crude leaf extracts of <i>Nicotiana benthamiana</i> containing recombinant foot and mouth disease virus-like particles are immunogenic in mice.
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