High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect.

We also detected an extremely significant correlation between N-glycan multiplicity and N-glycopeptide levels ( Figure 4B , Mann-Whitney test, p-value<0.0001).

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In contrast, En1-Cre; Srd5a3 fl/- mice showed a severe and highly significant (p=0.0007) motor coordination defect assessed with the rotarod test ( Figure 1G ).

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By contrast, the neurite defect is only close to significance on Laminin coating.

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