Tracing the transitions from pluripotency to germ cell fate with CRISPR screening.

However, by d6 we observed a highly significant depletion of PGCLC in multiple mutant lines (WT 43.1% ± 3.5; KO 8.9% ± 2.8), suggesting the effects of Zfp296 abrogation on PGCLC are progressive and manifest after specification (Fig. 4b ).

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