The results in Figure 1 showed the existence of a critical length for B1c (>27 bp) at which the TTP was significantly reduced (highlighted in blue). Two highly significant clusters were obtained by performing k-means cluster analysis and ANOVA test of sbLAMP results of WT and MT templates ( P > F = 4.44 × 10 –15 and P > F = 1.53 × 10 –14 , respectively).
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Allele-Specific Isothermal Amplification Method Using Unmodified Self-Stabilizing Competitive Primers.
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