Quantification of the NF-κB-p65 nuclear mean integrated density clearly validated these dynamics by showing a highly significant increase in nuclear NF-κB-p65 fluorescence after 30 minutes (93,96% ± 6,04%) and 1 hour (88,00% ± 12,00%) of TNF-α-treatment compared to untreated controls (<20%) (Fig. 4b ).
1
—
—