highly significantp value < 0.000001
Validation of RNA sequencing results and identification of potential biomarkers A total of 29 genes with highly significant differential expression in the RNA sequencing analysis (Table 4 , FDR-adjusted p value < 0.000001 and FC > 4 or FC < − 4) were selected for further real-time PCR analysis (ABI7900HT Fast Real-Time PCR System, Life Technologies).