Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in <i>Pichia pastoris</i> and Evaluation of Antitumor Activity In Vivo.

p < 0.05 and p < 0.01 were considered to be statistically significant and highly significant, respectively.

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The current research showed that the highest yield was achieved at a concentration of 1% and, when methanol concentration was increased to 1.5–3%, a decreasing trend of production was observed.

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