Consensus guidelines for the use and interpretation of angiogenesis assays.

Although the positioning of major vessels (e.g., dorsal aorta, cardinal vein, and intersegmental vessels in the trunk, or basilar artery in the head) is relatively invariant in normal animals at given stages, the precise location and paths taken by many smaller and/or later forming vessels (e.g., the central arteries of the hindbrain) are less stereotypic, and minor changes in the patterning of these vessels may not be significant.

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The variables, however, often largely unbeknownst to the user, lead to highly significant differences in results and a striking diversity in observations and conclusions.

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