highly significantP = 3.27 × 10 − 4
The difference between heterotrophic and autotrophic free energy of the 5′UTR was highly significant ( P = 3.27 × 10 − 4 ) with median values of − 2.1 kcal/mol and − 3.4 kcal/mol for increased and decreased TE, respectively (Additional file 12 : Figure S2b).