Optimization of the Ultrasonic-Assisted Extraction of Bioactive Flavonoids from Ampelopsis grossedentata and Subsequent Separation and Purification of Two Flavonoid Aglycones by High-Speed Counter-Current Chromatography.

According to ANOVA statistical data ( Table 2 ) and Pareto chart of the effects for the yield of the total flavonoids ( Figure 3 ), the quadratic terms of methanol concentration (X 1 ) and the liquid/solid ratio (X 3 ) had extremely significant effects on the extraction yield of TF.

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In addition, the high coefficient of determination R square values and the values of insignificance of lack of fit ( p > 0.05) were observed in Table 2 , indicating that the quadratic models were highly significant to the obtained data and capable of describing the relationship between the extraction conditions and responses during the UAE process.

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