While the quantified concentration of indigoidine via a colorimetric assay did not show a strong dependence on initial concentration of sugar present in the cultures grown in glycerol, a slight trend towards more intense coloration of the culture with increasing sugar concentration was observed (Fig. 3 a).
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Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.
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