a statistical trendp = 0.1732
Upregulation of mir-29a-5p and downregulation of mir-187-3p in stressed animals indicated by sequencing was confirmed by qPCR (two-tailed one sample t-tests, mir-29a-5p: t (11) = 4.207, p = 0.0015; mir-187-3p: t (11) = 3.751, p = 0.0032) and the other two miRNAs, mir-199a-5p and mir-135b-3p, showed a statistical trend in the expected direction based on the sequencing results (two-tailed one sample t-tests- mir-199a-5p: t (11) = 1.457, p = 0.1732; mir-135b-3p: t (10) = 1.623, p = 0.1357).