highly significantP < 0.001
In contrast to the highly significant ( P < 0.001) ANO1 current reduction (Fig. 4a ), SR Ca 2+ release was indistinguishable ( P > 0.05) from control scrambled siRNA expressing myotubes (Fig. 4b ), indicating that the observed ANO1 current reduction cannot be attributed to putative siRNA off-target effects hampering SR Ca 2+ release.