The results indicated that all the quadratic terms and liner terms as the ratio of liquid and solid (X 1 ) and ethanol concentration (X 4 ) and the interaction between X 1 X 3 , X 2 X 3 , were highly significant ( p ˂ 0.01) on the total flavonoids yield (Y 1 ), whereas the linear and quadratic effects of all independent variables were found to be significant ( p ˂ 0.05) on ABTS (Y 2 ) and DPPH (Y 3 ) radical-scavenging capacity of extracts from alfalfa ( Table 3 ).
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Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method.
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The second-order model developed for the yield of flavonoids from alfalfa was extremely significant.