Microglia are effector cells of CD47-SIRPα antiphagocytic axis disruption against glioblastoma.

To rule out direct inhibitory effects of anti-CD47 on glioma cells, we performed an in vitro proliferation assay and found no impairment of cell growth upon anti-CD47 treatment in two different glioma lines (T387, P = n.s.; T3832, P = n.s.), whereas the same concentration of anti-CD47 led to a highly significant increase of in vitro phagocytosis of four different glioma lines when cocultured with bone marrow-derived macrophages of NSG- Ccr2 RFP/wt -Cx3cr1 GFP/wt (T387, P = 0.003; T4121, P < 0.0001; T3832, P = 0.002; and T3691, P = 0.002) ( SI Appendix , Fig.

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