a nonsignificant trendP = 0.068
We found no difference in the protein levels of MFN1 ( P = 0.753) and MFN2 ( P = 0.852) between all groups, while OPA1 exhibited a nonsignificant trend to decrease in the high glucose group compared with the normal group( P = 0.068) but increase in the high glucose plus group compared with the high glucose group ( P = 0.064) (Figure S2 ). 3.6.