Highly significant correlation (R 2 = 0.94) between the FPKM and 2 -ΔΔCt values of these 27 single-copy genes ( Fig 4 , Table F in S1 File ) demonstrated the reliability of the RNA-seq data in our study.
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Fine mapping of the major QTL for seed coat color in Brassica rapa var. Yellow Sarson by use of NIL populations and transcriptome sequencing for identification of the candidate genes.
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