Non-myeloablative busulfan chimeric mouse models are less pro-inflammatory than head-shielded irradiation for studying immune cell interactions in brain tumours.

At 12 weeks, post-BM transplant, an overall trend of increased pro-inflammatory gene expression Il1b , Tnfa and Il6 was observed in the HIR transplant group (Fig. 2 b).

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Spleen samples in the HIR group showed a significant upregulation of Tnfa expression ( p < 0.05) relative to NMC and an increasing trend in comparison to both control and FC groups (Fig. 1 c).

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