Metabarcoding free-living marine nematodes using curated 18S and CO1 reference sequence databases for species-level taxonomic assignments.

Ecol Evol · 2019 · PMC6374678 · PMID 30805154

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a decreasing trendno p-value reported

UClust _ref and USearch9 produced contrasting OTU abundance patterns in the ribosomal reads; the number of OTUs in the former increased with clustering threshold, whereas in the latter, these followed a decreasing trend from maximum at 80% to minimum at 97%.

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