a decreasing trendP = 0.116
The absorbance pattern of the pigment 1 mixture was irregular at λ max = 520 nm and did not show any significant difference during the whole period of color shifting ( F = 0.362, df = 12, 26, P = 0.966; Figure 3A ); on the other hand, the absorbance pattern of the pigment 2 mixture showed a decreasing trend 12 h after starvation at λ max = 483 nm ( F = 1.737, df = 12, 26, P = 0.116; Figure 3B ), which revealed that pigment 2 mixture might be responsible for the color shift.