a statistical trendp < 0.001
In the PVN, CNO and ANG II significantly increased Fos staining in CTRL rats and there was a statistical trend for decreased Fos staining in the G i DREADD + CNO + ANG II as compared to CTRL + CNO + ANG II ( F (3,24) = 18.302, p < 0.001, one-way ANOVA; Tukey’s post hoc analysis, p = 0.084).