Network Walking charts transcriptional dynamics of nitrogen signaling by integrating validated and predicted genome-wide interactions.

Lastly, for TGA1, a well-studied TF in N signaling 26 – 28 , we observed a large and highly significant overlap (600 genes, p value = 1.78E−19, Fisher’s exact test) of direct regulated targets identified in root cells using the TARGET system, compared to DE genes resulting from TGA1 overexpression in roots of whole plants (Supplementary Data 4 and Supplementary Fig. 7 ) (see Methods).

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