highly significantP = 0.004
marginally significantP = 0.060
Phylogenetic generalised least squares regression The phylogenetic generalised least squares (PGLS) analyses on the Bulbophyllum backbone phylogeny revealed a generally strong, either marginally significant ( R 2 = 0.883, P = 0.060) or highly significant ( R 2 = 0.993, P = 0.004) relationship of, respectively, the stem and crown ages of the four lineages with their extant species numbers.