Identification and validation of reference genes for real-time quantitative RT-PCR analysis in jute.

By contrast, when 28S was used, the expression of ERF7B gradually increased at 4 h, increased to its highest level at 8 h, rapidly decreased at 12 h, and then continued a decreasing trend at 24 h (Fig. 6 b).

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The relative expression of ERF7B increased at 2 h, followed by a sharp accumulation at 4 h, an increasing trend at 8 h, down regulation at 12 h and a decreasing accumulation at 24 h when normalized by ACT and ACT7 + PP2Ac (Fig. 6 b).

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