highly significantP < 5 × 10 –4
The generated explanatory model showed that across all four responses, the highly significant ( P < 5 × 10 –4 ) factors and interactions were the ON/OFF stability (ΔΔ G ) of the anti-RBS-RBS hairpin, the interaction between TIR and hairpin ΔΔ G , TIR, and IPTG concentration ( Supplementary Table 6 ).