All the DNA methylation contents of these CG sites validated by the bisulfite pyrosequencing method, which disclosed the methylation contents of 3–5 CG sites in a primer set, showed highly significant linkage of CG methylation contents among the CG sites studied ( p < 0.001).
The results showed that LMO2_E148 CG and LMO2_P794 methylation levels higher than 28.5 and 29.5% at the newborn stage indicated a significantly higher risk of developing asthma ( p = 0.003 and p = 0.05, respectively), whereas IL10_P325 CG methylation level higher than 38.5% showed borderline significance ( p = 0.067) in the association of asthma development; there was no significant difference on the asthma development between GSTM1_P266 CG methylation levels higher and lower than 41.5% ( Table 2 ).