borderline significanceP =0.054
In agreement with our RNA-seq data, Glut1 staining was reduced, although with borderline significance ( P =0.054) in the Axin1 iEC − OE mice, but Claudin-5 staining was significantly reduced in Axin1 iEC − OE mice, suggesting that the elevated mRNA level seen at E11.5 in these mice might be a compensation mechanism.