highly significantp ‐value = 5.4 × 10 −50
Overall, population differentiation between basins along the first eigenvector was highly significant ( p ‐value = 5.4 × 10 −50 : summed across AMOVA comparisons). 3.4 Isolation and migration analysis reveals gene flow between divergent populations The unconstrained model, M‐mode in IMa2p, estimated a population splitting time parameter t of 0.29 (HiPt = 0.299, HPD95Lo‐Hi: 0.253–0.351).