Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on <i>Volvariella volvacea</i> at Low Temperature.

Based on quantitative results for MD gene expression ( Figure 4(a) ), levels in the V23 strain, which is not tolerant to low-temperature treatment, exhibited a decreasing trend after cells were subjected to low-temperature stress, and this low expression was maintained for 6 h of treatment.

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volvacea mycelia, first exhibited a decreasing trend and then an increasing trend during low-temperature treatment, suggesting that synthesis of mannitol was inhibited at the initial stage of treatment.

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