2 Although, MSCs were first isolated in bone marrow, 3 a decline is observed in using bone marrow MSCs while interestingly, an increasing trend is evident regarding exploration of various postnatal tissues as a source of MSCs. 4 Growing evidence suggests a remarkable regenerative potential of MSCs from dental derived tissue such as Dental pulp stem cells (DPSCs) from impacted third molar, 5 stem cells from apical papilla (SCAP) 6 and stem cells from human exfoliated deciduous teeth (SHED). 7 Permanent and deciduous (primary) teeth are considered as an easily accessible source for isolation and subsequent expansion of dental pulp cells for the purposes of tissue engineering.
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Isolation and culture of dental pulp stem cells from permanent and deciduous teeth.
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