highly significantP= 1.5 x 10 -8
It was not part of any batch effect, it was detected by three methods, there was a statistically highly significant level of agreement between 16S amplicon sequencing and both metagenomics ( P= 1.5 x 10 -8 ) and a targeted PCR/qPCR assay ( P =9.7 x 10 -21 ), none of 47 negative controls analysed by 16S sequencing were positive for S. agalactiae, and there was no association with mode of delivery ( Extended Data Fig. 7 ).