Owing to this mechanism, the BMSC/PRF construct formed a coherent whole, rather than two membranes stacked together. Determination of favorable hydrostatic pressure conditions for BMSC proliferation and chondrogenic differentiation in BMSC/PRF co-culture system Real-time quantitative PCR analysis showed an overall trend of proliferation and chondrogenic gene expression in the BMSC sheets co-cultured with PRF and stimulated under different pressure conditions.
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A novel construct with biomechanical flexibility for articular cartilage regeneration.
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