<i>Pax9</i> is required for cardiovascular development and interacts with <i>Tbx1</i> in the pharyngeal endoderm to control 4th pharyngeal arch artery morphogenesis.

Genotype analysis of 168 pups at weaning revealed that only two Tbx1 +/– ;Pax9 +/– mice were alive, a highly significant deviation from the 42 expected of each genotype from this cross ( P =1.4×10 −12 ; Table S2 ).

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