Pooling the unirradiated and 2 Gy-irradiated data indicated a highly significant TRAM-34-mediated depolarization of the membrane potential in K Ca 3.1 WT ( p = 0.005, n = 2 tests) but not in in the pooled K Ca 3.1 KO cells ( p = 0.8, n = 2 tests, data not shown).
As a result, and as indicated in Figure 1 J, the TRAM-34-mediated depolarization of the membrane potential differed almost significantly (p = 0.065, n = 4 pairwise comparison) between irradiated WT and K Ca 3.1 KO cells ( Figure 1 J).
In particular, TRAM-34 depolarized the membrane potential in unirradiated ( p = 0.074, one-sample t-test against 0, Bonferroni-corrected for n = 4 tests) and 2 Gy-irradiated KCa3.1 WT cells ( p = 0.055, n = 4 tests) in an almost significant manner.